Investigator(s)
| WHO Technical Officer |
Mohammad Ahmad WCO India- Research Mohammad Ahmad 
|
| Principal Investigator |
Bijayini Behera All India Insitute of Medical Sciences Bhubaneswar Bijayini Behera
|
Title(s) and abstract
| Scientific title | Population based Seroprevalence Study of Melioidosis in Odisha |
| Public title | Population based Seroprevalence Study of Melioidosis in Odisha |
| Background | Melioidosis, caused by the environmental saprophyte Burkholderia pseudomallei has substantial impact on the health-care systems and economies of numerous low and middle-income countries around the world, with counties of South Asia contributing to 44% of the global burden of the disease. Melioidosis so far is not included in the WHO portfolio of neglected tropical diseases (NTD), and thus research in melioidosis substantially lags behind other NTDs with similar global diseases burden. Seroprevalence studies in the general population are deemed necessary for an estimate of melioidosis endemicity as well as to explore the associated risk factors. Asymptomatic seroconversion is the most common event following environmental exposure and measurement of the antibodies can provide important epidemiological information and has public health significance. In India, few coastal southern states and union territories like Karnataka, Tamilnadu and Puducherry have undertaken community based and hospital based melioidosis seroprevalence studies. |
| Objectives | 1. To determine the age, sex and region stratified seroprevalence rate of B. pseudomallei (Melioidosis) in residents of Odisha in the age group of 5-60 years using Indirect Haemagglutination assay (IHA) 2. To determine risk factors for Melioidosis infection in Odisha state |
| Study Methods | Study design: Population based cross-sectional study of seroprevalence against melioidosis in the state of Odisha. Seropositivity will be defined as an IHA titre ≥20. Study Setting: The proposed study will be carried out in the state of Odisha. Study Population: The study will be carried out among persons 5-60 years’ of age, residing in the six randomly selected districts (under three Divisions) of state of Odisha, as aforementioned. Inclusion criteria: o Residents of Odisha. Standard definition of " resident" defined for the purposes of National population register as a person who has resided in a local area for the past six months or more will be used. o Participants 5-60 years’ age group Sample size and sampling strategy: Total sample size will be 1920. A multistage cluster sampling strategy like National Family Health Survey will be followed to select the primary sampling units. Field implementation: After explanation of the study and written consent, all the participants will be interviewed using a pre-designed and pre-tested questionnaire tool as attached. About 5 ml of blood sample will be collected by venipuncture. Laboratory testing of samples: The serum samples will then be analyzed for the presence of anti-B. pseudomallei antibodies using (IHA) test using the polysaccharide antigens. Data Analysis: Descriptive statistics and normality assessment of the data will be done before primary analysis. Categorical variables will be presented as proportion, and if required, with 95% confidence interval. Continuous data will be presented as mean (± Standard deviation) or median (± Inter quartile range) depending on the distribution of data. |
| Expected outcomes and use of results | This proposed study will add valuable perspectives to the current understanding of melioidosis in Odisha. As the present study also includes children, it will inform knowledge on the extent and probable route of this bacterial exposure. The three revenue divisions of Odisha covered under this study represent three different types of geographical and climatic set ups. The results of this study will provide valuable information about the different attributing risk factors for this disease. The burden of the disease in a particular state/locality can be ascertained by serological studies. It’s extremely important to establish endemicity so health professionals can be made aware of the existence of melioidosis as it often presents with non-specific symptoms. The result and outcome of the study will be shared and disseminated at state as well as national level for programmatic policy and intervention. |
| Keywords | Melioidosis, Odisha, NTDs, Seroprevalence |
Research Details
| Student research | No |
| Start Date | 01-Jul-2023 |
| End Date | 31-Dec-2023 |
| Key Implementing Institution | All India Institute of Medical Sciences Bhubaneswar (AIIMS-B) |
| Multi-country research | No |
| Nationwide research | No |
| India | |
| Research Domain(s) | Communicable Disease Research |
| Research field(s) | Infectious Disease |
| Involves human subjects | Yes |
| Population-Based Survey | |
| Data Collection | Primary data |
| Proposal reviewed by other Committee | Final decision available |